New publication explores methods for rapid characterization of Monoclonal antibodies

By September 2, 2020 No Comments

Monoclonal antibodies (mAbs) are important therapeutic glycoproteins, but their large size and structural complexity make them difficult to rapidly characterize. In a recent publication, Jean Lodge et al. present the first application of Activated ion electron transfer dissociation (AI-ETD) to mAb sequencing. For the standard NIST mAb, they found a high degree of complementarity between fragments generated using standard ETD with a short reaction time and AI-ETD with a long reaction time. Most importantly, AI-ETD revealed disulfide-bound regions that have been intractable, thus far, for sequencing with top-down MS.

Read the full article, Top-Down Characterization of an Intact Monoclonal Antibody Using Activated Ion Electron Transfer Dissociation, in Analytical Chemistry.

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