Trujillo et al (2019) published an article on maximizing tandem mass spectrometry acquisition rates for shotgun proteomics in a recent issue of Analytical Chemistry.
While advances in mass spectrometry (MS/MS) have lead to increased performance in shotgun proteomics experiments, ion trap scan duration is highly variable and often depends on the mass of the precursor.
Looking into this variability, the authors compared the performance of various static mass-to-charge ratio scan ranges for ion trap MS/MS acquisition to conventional dynamic mass-to-charge ratio scan ranges. Compared to the standard dynamic approach, the fixed mass-to-charge ratio scan range generated 12% more MS/MS scans and identified more unique peptides.
